UFR de Physique

Propositions de stages en laboratoire -- M2

Les offres sont actualisées en mai. Par exemple, les offres de stages pour l'année universitaire 2015-2016 seront mises en place en mai 2015, les offres de stages pour l'année universitaire 2016-2017 seront mises en place pour en mai 2016, etc.

Kinetic models of DNA damage-repair pathways by coupled rate equations

  • Option Finalisée « Physicien des hopitaux » du parcours Physique Biologique et Médicale
  • Laboratoire: Institut d'Electronique, Micro-électronique et Nanotechnologie (IEMN)
  • Responsable du stage: Fabrizio Cleri (fabrizio.cleri@univ-lille1.fr, )
  • Mots clés: Modelisation; equations differentielles; cycle cellulaire; reparation de defauts de l'ADN

Radiation therapy, as well as many of the commonly used cancer chemotherapeutic drugs, target DNA for cytotoxicity, chiefly by creating double-strand breaks in the molecule backbone. Such breaks can lead to chromosomal aberration and cell arrest, or cell death. The DNA damage response (DDR) to such cancer treatments in both malignant and normal cells/tissues determines the type of clinical treatment. The DDR is a complex set of cell processes involving multiple DNA repair, cell cycle regulation, and cell death/survival pathways (or networks) with both damage specificity and coordination of the DDR to different types of DNA damage (including the naturally occurring damage from various sources, which the cells need to repair daily to ensure survival). Over the last decade, significant progress has been made in elucidating these complex cellular and molecular networks involved in the DDR in human tumor and normal tissues.

Based on what has been learned about these processes using experimental in-vitro and in-vivo models, at the IEMN-NAMASTE laboratory we are building mathematical models of the DDR and DNA repair pathways, involved in determining the cytotoxicity to several classes of chemotherapy drugs and ionizing radiation. At this initial stage such models are still quite simple, and are set in the form of coupled partial differential equations (PDE). These sets of equations are supposed to describe the cell response to different levels and types of damage, according to the concentrations and reaction rates of the different proteins involved in the DDR. The PDE models of cellular-level response will be eventually embedded in our development code MODCEL, describing the evolution of fully coupled multicellular systems subject to different types of cytotoxic treatments (at this time, we are mostly focusing on radiotherapy treatments).

The successful candidate for this undergraduate internship (Stage M2) should have a good disposition toward theory and modelling of physical systems, and basic knowledge of biophysics. Computer programming (Fortran90, C, C ) may be desirable, but not a mandatory requirement.